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Image Search Results
Journal: Open Life Sciences
Article Title: α-Fetoprotein contributes to the malignant biological properties of AFP-producing gastric cancer
doi: 10.1515/biol-2022-0476
Figure Lengend Snippet: The relationship between AFP and Met. a: Knockdown of AFP reduces the c-Met expression, while the AFP overexpression in GCIY human GC cells elevates the c-Met expression. The AFP-siRNA reduces the expression of c-Met. The c-Met expression is significantly increased when AFP is overexpressed. b: Western blot showing overexpression of AFP in GCIY cells markedly induced the expression of c-Met in comparison with cells transfected with an empty GV230 vector.
Article Snippet: The
Techniques: Knockdown, Expressing, Over Expression, Western Blot, Comparison, Transfection, Plasmid Preparation
Journal: Scientific Reports
Article Title: p70S6K/Akt dual inhibitor DIACC3010 is efficacious in preclinical models of gastric cancer alone and in combination with trastuzumab
doi: 10.1038/s41598-023-40612-9
Figure Lengend Snippet: Two PIK3CA -mutated cell lines sensitive to growth inhibition by DIACC3010, HGC-27 and IM95m, had relatively low levels of pERK protein expression. ( a ) Thirteen GC cell lines were treated with DIACC3010 for 72 h (h) and then total cellular lysates were then immunoblotted, as indicated on the left. Black-edged rectangles delineate the blot. The 50% growth inhibition (GI 50 ) sensitivity to DIACC3010 is shown in the top row. ( b ) Heat map depiction of band intensities of the western blots, where green and red represent relatively low and high protein expression, respectively. Relative protein expression was normalized to total protein (for phosphor-proteins) and to β-actin. ( c ) GI 50 values versus phospho:total protein ratios for PAM pathway elements with corresponding correlation coefficients (R) and p values for the 13 GC cell lines. Statistical correlations were not significant ( p > 0.05). Original blots are presented in Supplementary Fig. .
Article Snippet:
Techniques: Inhibition, Expressing, Western Blot